Yuvraj made his Test debut against New Zealand in Mohali in late 2003 in front of his home crowd in Punjab due to the absence of regular captain Sourav Ganguly in the No. 6 position.He was omitted upon Ganguly's return but got another opportunity in the Test team in the 2004 Tour to Pakistan, when Ganguly was again injured. He scored his first Test century in a losing effort against Pakistan in the second Test at Lahore.
When Ganguly returned for the third Test, Akash Chopra was dropped and it appeared that the Indian selectors wanted to give him a regular place in the team. He was subsequently played as Virender Sehwag's opening partner, but after two poor matches in the Border-Gavaskar Trophy against Australia, he was dropped in favour of Gautam Gambhir.
He was recalled to the Test team, batting in the No. 6 position for the away series against Zimbabwe in August 2005 after Sachin Tendulkar was sidelined with tennis elbow. He then retained his position in the Test team after Ganguly was axed following a row with coach Greg Chappell. He scored a second Test century in the third and final Test in Pakistan in the series in January 2006, which India again lost. However, Yuvraj has struggled subsequently in 2006, failing to pass 50 in six Tests against England (two, both at home) and the West Indies (four, all away from home). His injury in late 2006 allowed the return of former captain Ganguly to the team, who subsequently top-scored in the series.
Yuvraj was included in India's Test squad to face Pakistan in November 2007, but was not included in the playing 11 for the first test. Yuvraj was picked in the 3rd Test due to an injured Sachin Tendulkar. India were 61/4 before he and Sourav Ganguly made a 300 run partnership with Yuvraj recording his highest Test score of 169.
He had a very poor Test series against Australia in Indian tour to that country in 2007–08. After his poor showing in the first two Tests he was dropped for the remainder of the series.
Yuvraj scored 85* put on an unbroken partnership of 163 with Sachin Tendulkar to defeat England in the First Test at Chennai on 15 December 2008. It was the fourth highest successful run chase in history and the highest in India.
